In vivo DNA replication dynamics unveil aging-dependent replication stress

Giacomo G. Rossetti, Noe ̈ lle Dommann, Angeliki Karamichali, Vasilis S. Dionellis, Ainhoa Asensio Aldave, Tural Yarahmadov, Eddie Rodriguez-Carballo, Adrian Keogh, Daniel Candinas, Deborah Stroka and Thanos D. Halazonetis Cell 187,6220–6234 2024

USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication

Hannah L. Mackay, Helen R. Stone, George E. Ronson, Katherine Ellis, Alexander Lanz, Yara Aghabi, Alexandra K. Walker, Katarzyna Starowicz, Alexander J. Garvin, Patrick Van Eijk, Stefan A. Koestler, Elizabeth J. Anthony, Ann Liza Piberger, Anoop S. Chauhan, Poppy Conway-Thomas, Alina Vaitsiankova, Sobana Vijayendran, James F. Beesley, Eva Petermann, Eric J. Brown, Ruth M. Densham, Simon H. Reed, Felix Dobbs, Marco Saponaro & Joanna R. Morris Nature Communications volume 15, Article number: 8102 (2024)

Timely lagging strand maturation relies on Ubp10 deubiquitylase-mediated PCNA dissociation from replicating chromatin

Javier Zamarreño, Sofía Muñoz, Esmeralda Alonso-Rodríguez, Macarena Alcalá, Sergio Rodríguez, Rodrigo Bermejo, María P. Sacristán & Avelino Bueno Nature Communications volume 15, Article number: 8183 (2024)

MCM2-7 loading-dependent ORC release ensures genome-wide origin licensing

L. Maximilian Reuter, Sanjay P. Khadayate, Audrey Mossler, Korbinian Liebl, Sarah V. Faull, Mohammad M. Karimi & Christian Speck Nature Communications volume 15, Article number: 7306 (2024)

Single-molecule characterization of SV40 replisome and novel factors: human FPC and Mcm10

Yujing Ouyang, Amani Al-Amodi, Muhammad Tehseen, Lubna Alhudhali, Afnan Shirbini, Masateru Takahashi, Vlad-Stefan Raducanu, Gang Yi, Ammar Usman Danazumi, Alfredo De Biasio, Samir M Hamdan Nucleic Acids Research, Volume 52 Pages 8880–8896 2024

Embryonic genome instability upon DNA replication timing program emergence

Saori Takahashi, Hirohisa Kyogoku, Takuya Hayakawa, Hisashi Miura, Asami Oji, Yoshiko Kondo, Shin-ichiro Takebayashi, Tomoya S. Kitajima & Ichiro Hiratani Nature volume 633, pages 686–694 (2024)

A high-throughput screening identifies MCM chromatin loading inhibitors targeting cells with increased replication origins

Lucia Falbo, Herve ́ Te ́ cher, Vincenzo Sannino, Michela Robusto, Giovanni Faga, Federica Pezzimenti, Francesco Romeo, Luca Gabriele Colombo, Stefania Vultaggio, Daniele Fancelli, Silvia Monzani, Valentina Cecatiello, Sebastiano Pasqualato, Mario Varasi, Ciro Mercurio and Vincenzo Costanzo iScience 27, 110567 2024

Unwinding of a eukaryotic origin of replication visualized by cryo-EM

Sarah S. Henrikus, Marta H. Gross, Oliver Willhoft, Thomas Pühringer, Jacob S. Lewis, Allison W. McClure, Julia F. Greiwe, Giacomo Palm, Andrea Nans, John F. X. Diffley & Alessandro Costa Nature Structural & Molecular Biology volume 31, pages 1265–1276 (2024)

SUMO protease and proteasome recruitment at the nuclear periphery differently affect replication dynamics at arrested forks

Kamila Schirmeisen, Karel Naiman, Karine Fréon, Laetitia Besse, Shrena Chakraborty, Anissia Ait Saada, Antony M Carr, Karol Kramarz, Sarah A E Lambert Nucleic Acids Research, Volume 52 Pages 8286–8302 2024

Sex-specific DNA-replication in the early mammalian embryo

Jason Alexander Halliwell, Javier Martin-Gonzalez, Adnan Hashim, John Arne Dahl, Eva R. Hoffmann & Mads Lerdrup Nature Communications volume 15, Article number: 6323 (2024)

Activity of DNA polymerase κ across the genome in human fibroblasts

Mariela C. Torres, Abbey Rebok, Dongxiao Sun, and Thomas E. Spratt https:// PNAS 121 (28) e2403130121 2024

Replication timing alterations are associated with mutation acquisition during breast and lung cancer evolution

Michelle Dietzen, Haoran Zhai, Olivia Lucas, Oriol Pich, Christopher Barrington, Wei-Ting Lu, Sophia Ward, Yanping Guo, Robert E. Hynds, Simone Zaccaria, Charles Swanton, Nicholas McGranahan & Nnennaya Kanu Nature Communications volume 15, Article number: 6039 (2024)

MCM8 interacts with DDX5 to promote R-loop resolution

Canxin Wen, Lili Cao, Shuhan Wang, Weiwei Xu, Yongze Yu, Simin Zhao, Fan Yang, Zi-Jiang Chen, Shidou Zhao, Yajuan Yang, and Yingying Qin EMBO J 43: 3044 - 3071 (2024)

Quantifying DNA replication speeds in single cells by scEdU-seq

Jeroen van den Berg, Vincent van Batenburg, Christoph Geisenberger, Rinskje B. Tjeerdsma Anchel de Jaime-Soguero, Sergio P. Acebrón, Marcel A. T. M. van Vugt & Alexander van Oudenaarden Nature Methods | Volume 21 1175–1184 2024

The loss of DNA polymerase epsilon accessory subunits POLE3–POLE4 leads to BRCA1-independent PARP inhibitor sensitivity

Hasan Mamar, Roberta Fajka-Boja, Mónika Mórocz, Eva Pinto Jurado, Siham Zentout, Alexandra Mihuţ, Anna Georgina Kopasz, Mihály Mérey, Rebecca Smith, Abhishek Bharadwaj Sharma, Nicholas D Lakin, Andrew James Bowman, Lajos Haracska, Sébastien Huet, Gyula Timinszky Nucleic Acids Research, Volume 52 Pages 6994–7011 2024

DNA replication in early mammalian embryos is patterned, predisposing lamina-associated regions to fragility

Shuangyi Xu, Ning Wang, Michael V. Zuccaro, Jeannine Gerhardt, Rajan Iyyappan, Giovanna Nascimento Scatolin, Zongliang Jiang, Timour Baslan, Amnon Koren & Dieter Egli Nature Communications volume 15, Article number: 5247 (2024)

Replication protein A dynamically re-organizes on primer/template junctions to permit DNA polymerase δ holoenzyme assembly and initiation of DNA synthesis

Jessica L Norris, Lindsey O Rogers, Kara G Pytko, Rachel L Dannenberg, Samuel Perreault, Vikas Kaushik, Sahiti Kuppa, Edwin Antony, Mark Hedglin Nucleic Acids Research, Volume 52 Pages 7650–7664 2024

Loss of POLE3-POLE4 unleashes replicative gap accumulation upon treatment with PARP inhibitors

Bethany Rebekah Hill, Meryem Ozgencil, Lauryn Buckley-Benbow, Sophie Louise Pamela Skingsley, Danielle Tomlinson, Carmen Ortueta Eizmendi, Alessandro Agnarelli,and Roberto Bellelli Cell Reports 43, 114205, 2024

Improved detection of DNA replication fork-associated proteins

Rebecca S. Rivard,Ya-Chu Chang, Ryan L. Ragland, Yee-Mon Thu, Muzaffer Kassab, Rahul Shubhra Mandal, Susan K. Van Riper, Katarzyna Kulej, LeeAnn Higgins, Todd M. Markowski, David Shang, Jack Hedberg, Luke Erber, Benjamin Garcia, Yue Chen, Anja-Katrin Bielinsky and Eric J. Brown Cell Reports 43, 114178 2024

Mechanism of DNA unwinding by MCM8-9 in complex with HROB

Ananya Acharya, Hélène Bret, Jen-Wei Huang, Martin Mütze, Martin Göse, Vera Maria Kissling, Ralf Seidel, Alberto Ciccia, Raphaël Guérois & Petr Cejka Nature Communications volume 15, Article number: 3584 (2024)

PrimPol Variant V102A with Altered Primase and Polymerase Activities

Elizaveta O. Boldinova, Andrey G. Baranovskiy, Yulia V. Filina, Regina R. Miftakhova, Yana F. Shamsutdinova, Tahir H. Tahirov, Alena V. Makarova Journal of Molecular Biology Volume 436 168542 2024

Parental histone transfer caught at the replication fork

Ningning Li, Yuan Gao, Yujie Zhang, Daqi Yu, Jianwei Lin, Jianxun Feng, Jian Li, Zhichun Xu, Yingyi Zhang, Shangyu Dang, Keda Zhou, Yang Liu, Xiang David Li, Bik Kwoon Tye, Qing Li, Ning Gao & Yuanliang Zhai Nature volume 627, pages 890–897 (2024)

RTF2 controls replication repriming and ribonucleotide excision at the replisome

Brooke A. Conti, Penelope D. Ruiz, Cayla Broton, Nicolas J. Blobel, Molly C. Kottemann, Sunandini Sridhar, Francis P. Lach, Tom F. Wiley, Nanda K. Sasi, Thomas Carroll & Agata Smogorzewska Nature Communications volume 15, Article number: 1943 (2024)

TopBP1 utilises a bipartite GINS binding mode to support genome replication

Matthew Day, Bilal Tetik, Milena Parlak, Yasser Almeida-Hernández, Markus Räschle, Farnusch Kaschani, Heike Siegert, Anika Marko, Elsa Sanchez-Garcia, Markus Kaiser, Isabel A. Barker, Laurence H. Pearl, Antony W. Oliver & Dominik Boos Nature Communications volume 15, Article number: 1797 (2024)

The genetic landscape of origins of replication in P. falciparum

Casilda Muñoz Castellano, Laurent Lacroix, Emilie Mathis, Paulina Prorok, Magali Hennion, Jose-Juan Lopez-Rubio, Marcel Méchali, Ana Rita Gomes Nucleic Acids Research, Volume 52 Pages 660–676 2024

DNA polymerase ε and δ variants drive mutagenesis in polypurine tracts in human tumors

Daria Ostroverkhova,Kathrin Tyryshkin, Annette K. Beach, Elizabeth A. Moore, Yosef Masoudi-Sobhanzadeh, Stephanie R. Barbari, Igor B. Rogozin, Konstantin V. Shaitan, Anna R. Panchenko,and Polina V. Shcherbakova Cell Reports 43, 113655 2024

Template switching between the leading and lagging strands at replication forks generates inverted copy number variants through hairpin-capped extrachromosomal DNA

Rebecca Martin , Claudia Y. Espinoza , Christopher R. L. Large, Joshua Rosswork, Cole Van Bruinisse, Aaron W. Miller, Joseph C. Sanchez, Madison Miller, Samantha Paskvan, Gina M. Alvino, Maitreya J. Dunham, M. K. Raghuraman, Bonita J. Brewer PLoS Genet 20(1): e1010850.

The proofreading exonuclease of leading-strand DNA polymerase epsilon prevents replication fork collapse at broken template strands

Tasnim Ahmad, Ryotaro Kawasumi, Tomoya Taniguchi, Takuya Abe, Kazuhiro Terada, Masataka Tsuda, Naoto Shimizu, Toshiki Tsurimoto, Shunichi Takeda, Kouji Hirota Nucleic Acids Research, Volume 51 Pages 12288–12302 2023

Single molecule MATAC-seq reveals key determinants of DNA replication origin efficiency

Anna Chanou, Matthias Weiβ, Karoline Holler, Atiqa Sajid, Tobias Straub, Jana Krietsch, Andrea Sanchi, Henning Ummethum, Clare S K Lee, Elisabeth Kruse, Manuel Trauner, Marcel Werner, Maxime Lalonde, Massimo Lopes, Antonio Scialdone, Stephan Hamperl Nucleic Acids Research, Volume 51 Pages 12303–12324 2023

DEK oncoprotein participates in heterochromatin replication via SUMO-dependent nuclear bodies

Agnieszka Pierzynska-Mach, Christina Czada, Christopher Vogel, Eva Gwosch, Xenia Osswald, Denis Bartoschek, Alberto Diaspro, Ferdinand Kappes, Elisa Ferrando-May J Cell Sci (2023) 136: jcs261329.

DONSON is required for CMG helicase assembly in the mammalian cell cycle

Cecile Evrin, Vanesa Alvarez, Johanna Ainsworth, Ryo Fujisawa, Constance Alabert, and Karim Labib EMBO rep 24: e57677 (2023)

RIF1 regulates early replication timing in murine B cells

Daniel Malzl, Mihaela Peycheva, Ali Rahjouei, Stefano Gnan, Kyle N. Klein, Mariia Nazarova, Ursula E. Schoeberl, David M. Gilbert, Sara C. B. Buonomo, Michela Di Virgilio, Tobias Neumann & Rushad Pavri Nature Communications volume 14, Article number: 8049 (2023)

Persistent Acetylation of Histone H3 Lysine 56 Compromises the Activity of DNA Replication OriginsR

Roch Tremblaya, Yosra Mehrjoo, Oumaima Ahmed, Antoine Simoneau, Mary E. McQuaid, El Bachir Affar, Corey Nislow, Guri Giaever & Hugo Wurtele Molecular and Cellular Biology Volume 43 Pages 566-595 2023

Replication‐induced DNA secondary structures drive fork uncoupling and breakage

Sophie L Williams, Corella S Casas‐Delucchi, Federica Raguseo, Dilek Guneri, Yunxuan Li, Masashi Minamino, Emma E Fletcher, Joseph TP Yeeles, Ulrich F Keyser, Zoë AE Waller, Marco Di Antonio and Gideon Coster EMBO J 42: e114334(2023)

The structural mechanism of dimeric DONSON in replicative helicase activation

Milos A. Cvetkovic1,Paolo Passaretti Agata Butryn Alicja Reynolds-Winczura, Georgia Kingsley, Aggeliki Skagia, Cyntia Fernandez-Cuesta, Divyasree Poovathumkadavil, Roger George, Anoop Chauhan, Satpal Jhujh, Grant S. Stewart, Agnieszka Gambus, and Alessandro Costa Molecular Cell Volume 83 P4017-4031 2023

Cooperative interaction of CST and RECQ4 resolves G‐quadruplexes and maintains telomere stability

Tingfang Li, Miaomiao Zhang, Yanjing Li, Xinyu Han, Lu Tang, Tengfei Ma, Xiaotong Zhao, Rui Zhao, Yuwen Wang, Xue Bai, Kai Zhang, Xin Geng, Lei Sui, Xuyang Feng, Qiang Zhang, Yang Zhao, Yang Liu, Jason A Stewart, and Feng Wang EMBO rep 24: e55494 (2023)

DONSON facilitates Cdc45 and GINS chromatin association and is essential for DNA replication initiation

Georgia Kingsley, Aggeliki Skagia, Paolo Passaretti, Cyntia Fernandez-Cuesta, Alicja Reynolds-Winczura, Kinga Koscielniak, Agnieszka Gambus Nucleic Acids Research, Volume 51, Pages 9748–9763

Function and mechanism of MCM8 in the development and progression of colorectal cancer

Shaojun Yu, Weixing Dai, Senlin Zhao, Yongzhi Yang, Ye Xu, Jianwei Wang, Qun Deng, Jinghu He and Debing Shi Journal of Translational Medicine (2023) 21:623

Synergism between CMG helicase and leading strand DNA polymerase at replication fork

Zhichun Xu, Jianrong Feng, Daqi Yu, Yunjing Huo, Xiaohui Ma, Wai Hei Lam, Zheng Liu, Xiang David Li, Toyotaka Ishibashi, Shangyu Dang & Yuanliang Zhai Nature Communications volume 14, Article number: 5849 (2023)

DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans

YISUI XIA, REMI SONNEVILLE, MICHAEL JENKYN-BEDFORD, LIQIN JI, CONSTANCE ALABERT, YE HONG, JOSEPH T. P. YEELES, AND KARIM P. M. LABIB SCIENCE Vol 381, Issue 6664

In silico protein interaction screening uncovers DONSON’s role in replication initiation

YANG LIM, LUKAS TAMAYO-ORREGO, ERNST SCHMID, ZYGIMANTE TARNAUSKAITE, OLGA V. KOCHENOVA, RHIAN GRUAR, SACHIKO MURAMATSU, LUKE LYNCH, AITANA VERDU SCHLIE,PAULA CARROLL, GEORGHE CHISTOL, MARTIN REIJNS, MASATU KANEMAKI, ANDREW JACKSON, AND JOHANNES C. WALTER SCIENCE Vol 381, Issue 6664

A dual role for the chromatin reader ORCA/LRWD1 in targeting the origin recognition complex to chromatin

Sumon Sahu, Babatunde E Ekundayo, Ashish Kumar, and Franziska Bleichert EMBO J 42: e114654 (2023)