4 June 2025

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The proofreading mechanism of the human leading-strand DNA polymerase ε holoenzyme Feng Wang, Qing He, Michael E. O’Donnell, and Huilin Li PNAS 122 (22) e2507232122

3 June 2025

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Compact Origins and Where to Find Them: ORC's Guide to Genome-Wide Licensing get actual link when it comes out Christian Speck, Luitpold Maximilian Reuter Bioessays. 2025 May 19:e70018.

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Most human DNA replication initiation is dispersed throughout the genome with only a minority within previously identified initiation zones Jamie T. Carrington, Rosemary H. C. Wilson, Eduardo de La Vega, Sathish Thiyagarajan, Tom Barker, Leah Catchpole, Alex Durrant, Vanda Knitlhoffer, Chris Watkins, Karim Gharbi & Conrad A. Nieduszynski  Genome Biology volume 26, Article number: 122 (2025)

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Cryo-EM structure of apo-form human DNA polymerase δ elucidates its minimal DNA synthesis activity without PCNA Yeonoh Shin ∙ Mark Hedglin ∙ Katsuhiko S. Murakami Journal of Biological Chemistry, Volume 301, Issue 4, 108342

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A conserved phosphorylation mechanism for regulating the interaction between the CMG replicative helicase and its forked DNA substrate Sandra Koit1 ∙ Nele Tamberg ∙ Allan Reinapae ∙ Lauri Peil ∙ Arnold Kristjuhan ∙ Ivar Ilves Journal of Biological Chemistry, Volume 301, Issue 4, 108408

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Effects of CDC45 mutations on DNA replication and genome stability Milena Denkiewicz-Kruk, Deepali Chaudhry, Alina Krasilia, Malgorzata Jedrychowska, Iwona J. Fijalkowska, Michal Dmowski Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Volume 1872, Issue 5, June 2025, 119936

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Multigenerational cell tracking of DNA replication and heritable DNA damage Andreas Panagopoulos, Merula Stout, Sinan Kilic, Peter Leary, Julia Vornberger, Virginia Pasti, Antonio Galarreta, Aleksandra Lezaja, Kyra Kirschenbühler, Ralph Imhof, Hubert Rehrauer, Urs Ziegler & Matthias Altmeyer  Nature.

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DNA replication timing reveals genome-wide features of transcription and fragility Francisco Berkemeier, Peter R. Cook & Michael A. Boemo Nature Communications volume16, Article number: 4658 (2025)

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USP37 prevents unscheduled replisome unloading through MCM complex deubiquitination Derek L. Bolhuis, Dalia Fleifel, Thomas Bonacci, Xianxi Wang, Brandon L. Mouery, Jeanette Gowen Cook, Nicholas G. Brown & Michael J. Emanuele  Nature Communications volume 16, Article number: 4575 (2025

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The yeast CST and Polα/primase complexes act in concert to ensure proper telomere maintenance and protection Kimberly Calugaru , Eun Young Yu , Sophie Huang , Nayim González-Rodríguez , Javier Coloma , Neal F Lue Nucleic Acids Research, Volume 53, Issue 7, 24 April 2025,

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Replicative DNA polymerase epsilon and delta holoenzymes show wide-ranging inhibition at G-quadruplexes in the human genome Suzanne E Hile , Matthias H Weissensteiner , Kara G Pytko , Joseph Dahl , Eduard Kejnovsky , Iva Kejnovská , Mark Hedglin , Ilias Georgakopoulos-Soares , Kateryna D Makova , Kristin A Eckert Nucleic Acids Research, Volume 53, Issue 8, 8 May 2025,

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Human DNA replication initiation sites are specified epigenetically by oxidation of 5-methyl-deoxycytidine Torsten Krude , Jiaming Bi , Rachel Doran , Rebecca A Jones , James C Smith Nucleic Acids Research, Volume 53, Issue 8, 8 May 2025,

13 May 2025

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DNA bending mediated by ORC is essential for replication licensing in budding yeast Wai Hei Lam, Daqi Yu, Qiongdan Zhang, and Shangyu Dang PNAS April 4, 2025 122 (14) e2502277122

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Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Emma E Fletcher, Morgan L Jones, and Joseph T P Yeeles EMBO J(2025)44: 2298 - 2322

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RIF1 controls replication timing in early mouse embryos independently of lamina-associated nuclear organization Tsunetoshi Nakatani ∙ Tamas Schauer ∙ Mrinmoy Pal ∙ Julia Zorn ∙ David M. Gilbert ∙ Maria-Elena Torres-Padilla Dev Cell

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Selective interactions at pre-replication complexes categorize baseline and dormant origins Bhushan L. Thakur, Christophe E. Redon, Haiqing Fu, Robin Sebastian, Nana A. Kusi, Sophie Z. Zhuang, Lorinc S. Pongor, Vilhelm A. Bohr & Mirit I. Aladjem Nature Communications volume 16, Article number: 4140 (2025)

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Chromatin-centric insights into DNA replication Yang Liu ∙ Zhengrong Zhangding ∙ Xuhao Liu ∙ Jiazhi Hu Trends in Genetics, Volume 41, Issue 5, 412 - 424

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The Depletion of TRAIP Results in the Retention of PCNA on Chromatin During Mitosis Leads to Inhibiting DNA Replication Initiation Yasushi Shiomi, Akiyo Hayashi, Yuichiro Saito, Masato T. Kanemaki, Hideo Nishitani Genes to Cells, 2025; 30:e70006

2 April 2025

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Z-form DNA-RNA hybrid blocks DNA replication Shiyu Wang, Yan Xu Nucleic Acids Research, Volume 53, Issue 5, 24 March 2025, gkaf135,

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A conserved phosphorylation mechanism for regulating the interaction between the CMG replicative helicase and its forked DNA substrate Sandra Koit, ∙ Nele Tamberg ∙ Allan Reinapae ∙ Lauri Peil ∙ Arnold Kristjuhan ∙ Ivar Ilves Journal of Biological Chemistry, Volume 0, Issue 0, 108408

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The Shu complex interacts with the replicative helicase to prevent mutations and aberrant recombination Adeola A Fagunloye , Alessio De Magis , Jordan H Little , Isabela Contreras, Tanis J Dorwart , Braulio Bonilla , Kushol Gupta , Nathan Clark , Theresa Zacheja, Katrin Paeschke and Kara A Bernstein EMBO J(2025)44: 1512 - 1539

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Cryo-EM structure of apo-form human DNA polymerase δ elucidates its minimal DNA synthesis activity without PCNA Yeonoh Shin ∙ Mark Hedglin ∙ Katsuhiko S. Murakami Journal of Biological Chemistry, Volume 301, Issue 4, 108342

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Transcription near arrested DNA replication forks triggers ribosomal DNA copy number changes Mariko Sasaki, Takehiko Kobayashi Nucleic Acids Research, Volume 53, Issue 3, 10 February 2025, gkaf014,

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Dual DNA replication modes: varying fork speeds and initiation rates within the spatial replication program in Xenopus Diletta Ciardo, Olivier Haccard, Francesco de Carli, Olivier Hyrien, Arach Goldar, Kathrin Marheineke Nucleic Acids Research, Volume 53, Issue 3, 10 February 2025, gkaf007,

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DNA polymerase zeta can efficiently replicate structures formed by AT/TA repeat sequences and prevent their deletion Mili Das, Suzanne E Hile, Jennifer Brewster, Jan Leendert Boer, Rachel Bezalel-Buch, Qiong Guo, Wei Yang, Peter M Burgers, Kristin A Eckert, Catherine H Freudenreich Nucleic Acids Research, Volume 53, Issue 3, 10 February 2025, gkae1254,

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Replication-IDentifier links epigenetic and metabolic pathways to the replication stress response Sophie C. van der Horst, Leonie Kollenstart, Amandine Batté , Sander Keizer, Kees Vreeken, Praveen Pandey, Andrei Chabes & Haico van Attikum Nature Communications | (2025)16:1416

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The double life of mammalian DNA replication origins Olivier Hyrien, Guillaume Guilbaud and Torsten Krude Genes & Dev. 2025.

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Nanopore sequencing reveals that DNA replication compartmentalisation dictates genome stability and instability in Trypanosoma brucei Marija Krasiļņikova, Catarina A. Marques, Emma M. Briggs, Craig Lapsley, Graham Hamilton, Dario Beraldi, Kathryn Crouch & Richard McCulloch Nature Communications volume 16, Article number: 751 (2025)

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Sequence specificity of an essential nuclear localization sequence in Mcm3 Ziyi Wang, Yun Jing Zhang, Qian-yi Zhang, Kate Bilsborrow, Matthew Leslie, Raymond T. Suhandynata, Huilin Zhou PLoS Genet 21(1): e1011499.

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Unidirectional MCM translocation away from ORC drives origin licensing Agata Butryn, Julia F. Greiwe & Alessandro Costa Nature Communications volume 16, Article number: 782 (2025)

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A tale of two strands: Decoding chromatin replication through strand-specific sequencing Zhiming Li ∙ Zhiguo Zhang Mol Cell Volume 85, Issue 2P238-261January 16, 2025

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Structural insight into Okazaki fragment maturation mediated by PCNA-bound FEN1 and RNaseH2 Yuhui Tian , Ningning Li , Qing Li , and Ning Gao gaon@pku.edu.cn EMBO J (2024) 44: 484 - 504

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Cell cycle progression of under-replicated cells Min Huang, Chang Yang, Litong Nie, Huimin Zhang, Dandan Zhu, Chao Wang, Jeong-Min Park, Mrinal Srivastava, Elina Mosa, Siting Li, Mengfan Tang, Xu Feng, Sarah J Keast, Fabio Stossi, Junjie Chen Nucleic Acids Research, Volume 53, Issue 1, 13 January 2025, gkae1311,

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H3K56 acetylation regulates chromatin maturation following DNA replication Shoufu Duan, Ilana M. Nodelman, Hui Zhou, Toshio Tsukiyama, Gregory D. Bowman & Zhiguo Zhang Nature Communications volume 16, Article number: 134 (2025)

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Telomere-to-telomere DNA replication timing profiling using single-molecule sequencing with Nanotiming Bertrand Theulot, Alan Tourancheau, Emma Simonin Chavignier, Etienne Jean, Jean-Michel Arbona, Benjamin Audit, Olivier Hyrien, Laurent Lacroix & Benoît Le Tallec Nature Communications volume 16, Article number: 242 (2025)

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MCM2-7 ring closure involves the Mcm5 C-terminus and triggers Mcm4 ATP hydrolysis Sarah V. Faull, Marta Barbon, Audrey Mossler, Zuanning Yuan, Lin Bai, L. Maximilian Reuter, Alberto Riera, Christian Winkler, Indiana Magdalou, Matthew Peach, Huilin Li & Christian Speck Nature Communications volume 16, Article number: 14 (2025)

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Repair of replication-dependent double-strand breaks differs between the leading and lagging strands Michael T. Kimble ∙ Aakanksha Sane ∙ Robert J.D. Reid ∙ Matthew J. Johnson ∙ Rodney Rothstein ∙ Lorraine S. Symington Mol Cell Volume 85, Issue 1P61-77.E6January 02, 2025

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Communication between DNA polymerases and Replication Protein A within the archaeal replisome Markel Martínez-Carranza, Léa Vialle, Clément Madru, Florence Cordier, Ayten Dizkirici Tekpinar, Ahmed Haouz, Pierre Legrand, Rémy A. Le Meur, Patrick England, Rémi Dulermo, J. Iñaki Guijarro, Ghislaine Henneke & Ludovic Sauguet Nature Communications volume 15, Article number: 10926 (2024)

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DNA replication initiation drives focal mutagenesis and rearrangements in human cancers Pierre Murat, Guillaume Guilbaud & Julian E. Sale Nature Communications volume 15, Article number: 10850 (2024)